Sand Cold Test
The Sand Cold Test gives a reasonable idea of emergence under less than ideal conditions, but not severe, conditions. Using only sand to cover seeds reduces variability that can come with the use of soil The variability comes from different types of soil, the amount of pythium present in the soil, and other factors. However eliminating soil can reduce the stress inflicted on the seeds making it a test that may better predict milder stressful conditions.
Saturated Cold Test
Test Details
Two replications of 100 seeds are placed on Versapak® that has been moistened and chilled overnight at 10C. The amount of water added will bring the sand used to cover the seeds to 70% of its water holding capacity. The germination cart is returned to 10C for seven days without light. The cart is moved to 25C for seven additional days (4-7 are an option). A quicker option is to for the cold period to be three days and the warm period four days.
Seedlings that emerge through the sand mixture are evaluated according to modified AOSA Rules For Testing Seeds. The modification reflects the need for stronger root and shoot development under stressful conditions. The use of sand & soil are recommended in the AOSA Seed Vigor Testing Handbook.