Bacterial Plating Tests

Test Details

Seeds are soaked in sample extraction buffer. Depending on seed type, seeds may also be ground. Serial dilutions of seed extract are plated onto agar media selective for the target pathogen(s). After incubation and visual examination, follow-up tests such as PCR or pathogenicity testing may be necessary.

Bacterial Plating Tests

Sample Requirements

Variable. Often 1000-5000 seeds for large seeds such as corn and cucurbits; 10,000 to 30,000 for small seeds such as pepper, tomato, lettuce.

Iowa State University Laboratory Seed Testing Price List