Soak Tests
Duration: Variable
Detection of nematodes or internal seed-borne fungi such as downy mildews and loose smut (Ustilago nuda).
Soak Tests
Test Details
Seeds are soaked overnight in water (nematodes) or in a dilute sodium hydroxide solution (downy mildews, loose smut). Nematodes are concentrated by sieving, followed by microscopic examination. For fungi, seed embryos are stained and examined microscopically.
Soak Tests
Sample Requirements
400 to 30,000 seeds, depending on plant species and test(s) requested.